HPLC sample vial storage

During the chromatographic test process, sample vials are an unavoidable topic in the experiment. In order to obtain accurate test data, we must ensure the cleanliness, integrity, and sealing of the vials. So how should we store the sample vials to ensure that the vials are not contaminated? Let's learn about it together through this article.

Storage conditions of HPLC sample vials.

About vial storage

Two things are most important for vial quality and performance of vials: temperature and humidity. After cleaning the sample vials by standard procedures, you can invert them to dry. Or, you can place them in a drying oven to remove all accumulated water. Or, you can blow dry them with nitrogen to remove any leftover moisture. Accumulated water is easy to breed bacteria and affect the test results. The best temperature for storing glass sample vials is 15-25°C. Avoid high heat or big temperature changes. They may cause the vials to break.

The sample vials need to be placed with the mouth of the bottle facing upwards to protect the mouth of the vial from wear and tear.

Dust prevention is also a crucial step. The factory packaging of our vials has a very good dust prevention effect. After putting them in the packaging, the vials can be prevented from contacting with dust and pollutants. So be sure not to throw away our packaging box after receiving the vials.

 Want to know how to Choose Crimp Vial vs. Snap Vial vs. Screw Cap Vial?, Check this article:Crimp Vial vs. Snap Vial vs. Screw Cap Vial, How to Choose?

Check the sample vials often. Make sure they are not damaged or dirty. Even if the vials are cleaned and stored in a standardized manner, the sample vials still need to be replaced regularly. Depending on the frequency of experimental testing, we recommend that you replace the sample vials every 1-3 months.

Standardized batch management of sample vials can achieve better storage effects. Classify by the vials' use time and the loaded samples. It will help you store the vials clearly and efficiently. If you need to store vials in batches, our sample vial rack is also a very good choice. Using a sample vial rack can prevent the risk of sample vials being broken and worn against each other.

Use different sample vials for different types of samples to avoid contamination caused by cross-use of vials. It is best to use a dedicated bottle for a dedicated purpose.

plastic vial

If you use plastic sample vials in your laboratory, be sure to avoid the plastic vials from being exposed to sunlight. Vials made of plastic will oxidize when exposed to ultraviolet rays for a long time, affecting the service life of the vials. 

                           Want to know the details of the Polypropylene Autosampler Vials please check this article: 9mm Screw Top Plastic Autosampler Vial

Septum & Cap Storage

Septum storage

Cap pads are also crucial for sample vials. But, most people ignore how to store them.

The septum is disposable and must be replaced in time after each injection test, so we often prepare a large number of septa in the laboratory.

The best storage temperature for PTFE/silicone septa is 15-25°C. Silicone should not be exposed to temperatures above 40°C or below 5°C for long. Such temperatures cause the material to deteriorate, crack, lose elasticity, and harm sealing.

Ensure the environment is dry and the humidity is controlled between 30-50%.

Silicone is very easy to age and should be avoided from sunlight. Ultraviolet rays will accelerate the aging of the material and should be placed in a light-shielded box or drawer. Avoid folding, especially septa with pre-cut slits. Folding may cause the septum to break, and folding will also cause PTFE and silicone to delaminate.

Stay away from sharp instruments, which may bruise the septum and cause inaccurate test data during the injection test.

                          For more about  HPLC Vial Caps and Septa,please check this article:For HPLC Vial Caps and Septa, you need to know

Cap storage

There are two types of small bottle caps: metal caps and plastic caps.

Both plastic caps and metal caps should be kept dry, clean and in a suitable environment.

It should be noted that the metal cap should be avoided from being squeezed, which will cause the cap to deform. Keep the environment dry. Metals are prone to rust in humid environments.

Replace the vial regularly

Storing sample vials in a standard way can greatly increase their service life. But, vials still need regular replacement. Lab staff must regularly check the vials' appearance. They must then decide if they need replacement based on how often they are used.